SLU-PP-332 is a synthetic small-molecule pan-agonist of the estrogen-related receptors ERRα, ERRβ and ERRγ, with the greatest reported potency at ERRα. The file slug sometimes appears as “SLU-PP-322,” but the compound described in the primary literature is SLU-PP-332.
What Are the Key SLU-PP-332 Research Specifications?
| Specification | Research record |
|---|---|
| Compound | SLU-PP-332 |
| Category | misc |
| Listed purity | >99% |
| Molecular weight | 290.32 g/mol |
| Intended use | Laboratory research only |
What is the chemical identity of SLU-PP-332?
SLU-PP-332 has formula C18H14N2O2 and average molecular mass 290.32 g/mol. It is not a peptide and its mass is not “variable” unless a different salt or solvate is supplied. The lot identity should be matched to the published structure by LC-MS and NMR, not by the product name alone.
How does SLU-PP-332 work?
ERRs are orphan nuclear receptors that coordinate oxidative metabolism and mitochondrial gene programmes, often with the coactivator PGC-1α. In cotransfection reporter assays, the original study reported pan-ERR agonism with strongest activity at ERRα. In C2C12 muscle cells, the compound increased mitochondrial respiration and induced genes associated with an acute aerobic-exercise response. Mouse experiments reported a shift toward oxidative type IIa fibres and increased endurance.
Those findings make SLU-PP-332 a pathway probe and preclinical tool, not proof that a small molecule reproduces every systemic adaptation to exercise. Later mouse work explored energy expenditure, fatty-acid oxidation and metabolic phenotypes; human efficacy and safety have not been established.
Which assays and controls are most informative?
Use receptor-specific reporter assays and ERRα-, ERRβ- or ERRγ-deficient cells to separate subtype contributions. Include a structurally unrelated ERR ligand and vehicle. Confirm proximal transcriptional responses before whole-cell respiration or differentiation endpoints. For Seahorse experiments, report cell number normalization, medium composition and mitochondrial stress-test parameters.
How should SLU-PP-332 be handled?
Prepare stocks from measured solubility for the verified neutral form and keep final vehicle concentration constant. Inspect aqueous dilutions for precipitation because nominal dose may exceed soluble exposure. Protect stocks from light, use single-use aliquots and establish solution stability by LC-MS. Quantitative work should use a reference standard and an internal standard rather than HPLC area percentage alone.
Which Sources Support SLU-PP-332 Research?
- Billon et al., identification of SLU-PP-332 and ERRα-dependent exercise-response signalling, ACS Chemical Biology (2023), PMID 36988910.
- Billon et al., metabolic phenotypes in obese mouse models, Journal of Pharmacology and Experimental Therapeutics (2024), PMID 37739806.
How Can SLU-PP-332 Results Be Interpreted Without Overclaiming?
The strongest SLU-PP-332 evidence connects identity to mechanism to phenotype in that order. For SLU-PP-332, chemical identity, target engagement and downstream phenotype are separate layers. A change in migration, viability or gene expression does not by itself prove the proposed molecular target. A useful study includes an orthogonal analytical identity check, a target-dependent perturbation and a second assay that measures the same biology by a different method. This design makes a positive result easier to reproduce and a negative result easier to interpret.
For citation-ready SLU-PP-332 reporting, provide raw concentration units, biological replicate counts, prespecified exclusions and the exact time point. Separate exploratory endpoints from confirmatory ones. If exposure was not measured, describe potency as nominal rather than intracellular or free concentration. Limit the conclusion to the model used; avoid converting a pathway observation into a claim about clinical benefit.
Is SLU-PP-332 for Research Use Only?
SLU-PP-332 is supplied for laboratory research only. It is not for human or veterinary use.
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What Are Common Questions About SLU-PP-332?
What is SLU-PP-332?
SLU-PP-332 is a synthetic small-molecule pan-agonist of the estrogen-related receptors ERRα, ERRβ and ERRγ, with the greatest reported potency at ERRα. The file slug sometimes appears as “SLU-PP-322,” but the compound described in the primary literature is SLU-PP-332.
How should researchers verify SLU-PP-332?
Confirm the exact molecular form on the lot certificate and pair chromatographic purity with an orthogonal identity method such as mass spectrometry or NMR. Use the molecular weight, counterion and content stated for that verified form when calculating molarity.
Is SLU-PP-332 intended for human use?
SLU-PP-332 is listed for controlled laboratory research only. It is not intended for human or veterinary use.